bisulphite是什么意思,bisulphite怎么读,
英式发音:[baɪˈsʌlfaɪt] 美式发音:[baɪˈsʌlfaɪt]
1.Bisulphite conversion of unmethylated cytosine residues followed by detection methods such as sequencing of distinct loci has become accepted as the gold standard for detecting 5-methylcytosines.
非甲基化胞嘧啶残基的亚硫酸氢盐转换,接着是检测方法,例如不同位点的测序已经被接受为检测5甲基胞嘧啶的金标准。
2.Bisulphite conversion of unmethylated cytosine residues followed by detection methods such as sequencing of distinct loci has become accepted as the gold standard for detecting 5-methylcytosines.
非甲基化胞嘧啶残基的亚硫酸氢盐转换,接着是检测方法,例如不同位点的测序已经被接受为检测5甲基胞嘧啶的金标准。
3.It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region.
它依赖于用亚硫酸氢钠处理后,非甲基化的 胞嘧啶选择性的去氨基为 尿嘧啶,通常接着所选的靶向区域的PCR扩增。
4.In order to clarify these controversial findings, we developed a new bisulphite sequencing protocol to determine the methylation status of p16.
为了阐明这些有争议的发现,我们采用了一种新的亚硫氰酸盐测序工具来确定。
5.Here, a detailed protocol for bisulphite conversion, primer design, and optimization of PCR conditions is given.
这里,给出了亚硫酸氢盐转换,引物设计和PCR条件优化的一个详细的实验方案。